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. 2018 Feb 21;8:3377. doi: 10.1038/s41598-017-17540-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LAMP2 is increased in calcifying rodent aortas. Rat aortic calcification was induced by subcutaneous injection with 1.2 × 10⁵ IU/kg/day vitamin D₃ or vehicle for 3 days. After total 14 days, aortas were dissected and total RNA was isolated. qPCR results revealed that LAMP2 was increased concomitant with osteogenic transformation showing increased RUNX2 and BMP2, and decreased TAGLN. Six rats for each group were analyzed. Values are presented as mean ± SEM. *P < 0.05.