Figure 1.

(a) Far- and near-UV spectra (of ~12 µM CaM or 30 µM, respectively) in the presence of equal amounts (400 µM) of saturating EGTA (black) or Ca²⁺ (red), and CaM previously incubated with 5.7 mgmL⁻¹ NPs (blue). (b) Thermal denaturation profiles of ~12 µM CaM previously incubated with 1.7 mgmL⁻¹ NPs (blue) and in the presence of saturating (120 µM) EGTA (black) and Ca²⁺ (red). θ₂₂₂ has been normalized to account for different starting values (θ_{222, normalized} = θ₂₂₂/θ_{222 at 20 °C}).