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. 2018 Feb 21;8:3420. doi: 10.1038/s41598-018-21571-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SPR analysis of CaM-NP interaction. (a) Example of sensorgram obtained by flowing NPs (0.1 mg/mL) on a flow cell, where 2400 RU of CaM were previously immobilized via amine coupling. Injection was performed for 600 s (flow rate 10 µL/min) and dissociation was followed for 1800 s. The red curve refers to data fitting using a single exponential function. The dashed grey line refers to injection of the same amount of NPs previously incubated with 1.6 µM CaM. (b) Example of dissociation curves obtained by SPR overlapped to the fitting curves according to a single exponential in the 0.1–0.5 mg/mL NP range.