Table 1.

Far-UV CD data: spectral shapes of apo CaM incubated with CaF₂ NP are very similar to those of Ca²⁺-loaded unbound protein.

	ϴ208	ϴ222	ϴ222/ϴ208	Δϴ222/ϴ222a
CaM Ca2+	−16.34	−16.29	1.00	21.7%
CaM EGTA	−14.15	−13.38	0.95
CaM + NPs	−15.74	−15.75	1.00	17.6%
CaM WT + NPs EGTA	−14.09	−13.39	0.95

^aRelative change in ellipticity (θ) at λ = 222 nm as obtained by the difference in θ in the presence of Ca²⁺ or in the apo conditions (EGTA), divided by θ in the apo conditions.