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. 2018 Feb 21;8:3419. doi: 10.1038/s41598-018-21183-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of rat dental pulp stem cells (rDPSCs). (a) Colony-forming capability of rDPSCs as shown by toluidine blue staining. Representative images of colony-froming unit fibroblats (CFU-Fs) in a culture dish (left panel) and fibroblastic colonies (right panel). (b) Representative image of passage 1 (P1) rDPSCs. (c) Representative image of rDPSCs with BrdU-positive nuclei. (d) Population doubling (PD) score of rDPSCs. (e,f) Immunophenotype assay by flow cytometric analysis. Red histograms: cell surface antigen-specific antibodies; blue histograms: subclass-matched control antibodies. Percentiles indicate the average for each antigen. PE: phycoerythrin (e). Percentiles of cell surface antigen-positive cells among total cells (n = 3 per group). Graph bars are the means ± standard error of the mean (SEM) (f). (g–i) Multipotency of rDPSCs. Odontogenic/osteogenic (g), chondrogenic (h), and adipogenic (i) capacity.