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. 2018 Feb 21;8:3419. doi: 10.1038/s41598-018-21183-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of the NO scavenger carboxy-PTIO on the odontogenic capacity of rDPSCs under stimulation with NOC-18. (a,d) Effects of carboxy-PTIO on ALP activity by ALP staining (a) and ALP activity test (d). (b,e) Effects of carboxy-PTIO on phosphate deposition by von Kossa staining (b) and measurement of the von Kossa-positive area (e). (c,f) Effects of carboxy-PTIO on calcium deposition by Alizarin Red-S staining (c) and measurement of calcium contents (f). (d,e,f) n = 3 per group; *p < 0.05. Graph bars are the means ± SEM.