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. 2018 Feb 21;8:3419. doi: 10.1038/s41598-018-21183-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of NOC-18 on iNOS expression, NO production, and tertiary dentin formation in vivo. (a) Expression of inducible nitric oxide synthase (iNOS) and nitrotyrosine (NT) in rDPSCs treated with NOC18 (10 μM) as assessed by double immunofluorescence. Merge: Merged images of iNOS and NT. (b–e) Effects of NOC-18 on tertiary dentin formation in the dentin-dental pulp complex under a tooth cavity. Localization of NT on days 1 (b) and 7 after tooth preparation by immunohistochemistry (c). ALP activity on day 3 by immunohistochemistry (d). Tertiary dentin formation on day 10 by hematoxylin and eosin (HE) staining (e).