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. 2018 Feb 21;8:3438. doi: 10.1038/s41598-018-21720-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of interstitial fibrosis after unilateral renal I/R injury at 7 days of reperfusion. Col1a2 (A), Col III (B) and TGFb (C) expression in ischemic murine kidneys. Detection of interstitial fibrosis by Massons’s trichrome staining in wildtype control (D), knockout control (E), wildtype with ischemia/reperfusion injury (F) and knockout mice with ischemia/reperfusion injury (G) as well as quantification of the results (H). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Col III = Collagen 3, TGFbeta = Transforming growth factor beta, KO = knockout, WT = wildtype, CTL = control, I/R = ischemia/reperfusion-injury, FC = foldchange. n = 6 mice in each group.