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. 2018 Feb 15;197(4):481–491. doi: 10.1164/rccm.201706-1139OC

Figure 9.

Figure 9.

Effect of supernatant of mucopurulent material (SMM) on airway surface liquid (ASL) height in non–cystic fibrosis (CF) versus CF human bronchial epithelial cultures. (A) Non-CF (normal) and CF human bronchial epithelial cultures were exposed to phosphate-buffered saline (PBS) or SMM for 72 hours. ASL height was evaluated as described in Methods, and is shown as the percentage increase after treatment with SMM over PBS for both groups. Data represent mean ± SD. n = 5–6. *P < 0.05, CF versus non-CF cultures. (B) Non-CF human bronchial epithelial cultures were exposed to SMM for 48 hours and ASL height was evaluated by XZ confocal microscopy. CFTRInh-172 was added apically as a dry powder in perfluorocarbon and ASL height was remeasured. ASL height is expressed as percentage inhibition of ASL height by CFTRInh-172 relative to ASL height from PBS- or SMM-treated non-CF human bronchial epithelia. Data represent mean ± SD. n = 4. *P < 0.05, SMM + CFTRInh-172 versus SMM. CFTR = CF transmembrane conductance regulator.