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(A) The cytotoxic effect of 5-Fu on the HCT8S and HCT8R cells. Cell viability was measured by MTT assay after 24 h treatment with 5-Fu. Data are mean ± SD from three independent experiments. (B) ABCB4 was the most significantly down-regulated among other resistance-related ABC family members. Data are mean ± SD from three independent experiments; **P<0.01. (C) Western blot revealed that ABCB4 was down-regulated in HCT8R cells.
