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. 2018 Feb 21;38(1):BSR20171428. doi: 10.1042/BSR20171428

Figure 2. Knockdown of ABCB4 decreases apoptosis to regulate the resistance in the HCT8S cells.

Figure 2

(A) Knockdown of ABCB4 significantly increased the chemoresistance of HCT8S cells, but had no effect on the cell viability without 5-Fu treatment. Cell viability was detected by MTT assay. Data are mean ± SD from three independent experiments; ***P<0.001. (B) The colony formation assay were performed with or without the treatment of 5-Fu after the knockdown of the ABCB4. (C) Cell apoptosis by flow cytometry analysis after Annexin V-FITC and PI double staining. Data are mean ± SD from three independent experiments; ***P<0.001. (D) The knockdown of ABCB4 was confirmed by Western Blot. Apoptosis-related proteins were detected by Western Blot with or without 5-Fu treatment.