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. 2018 Feb 21;38(1):BSR20171428. doi: 10.1042/BSR20171428

Figure 3. Overexpression of ABCB4 induces apoptosis to regulate the sensitivity in the HCT8R cells.

Figure 3

(A) Overexpression of ABCB4 significantly increased the sensitivity of HCT8R cells. Cell viability was detected by MTT assay. Data are mean ± SD from three independent experiments; *P<0.05. (B) The colony formation assay were performed with or without the treatment of 5-Fu after the overexpression of the ABCB4. (C) Cell apoptosis by flow cytometry analysis after Annexin V-FITC and PI double staining. Data are mean ± SD from three independent experiments; **P<0.01.***P<0.001. (D) The overexpression of ABCB4 was confirmed by Western Blot. Apoptosis-related proteins were detected by Western Blot with or without 5-Fu treatment.