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RT-qPCR verification of RNA-seq results. RNA-seq results for selected genes were verified using RT-qPCR. First-strand cDNA was synthesized from total RNA using random primers and RT-qPCR carried out in triplicate on 5-fold diluted cDNA. ΔΔC T values were normalized to those of housekeeping genes (up to 5 genes) to compare fold differences between samples.
