Skip to main content
. 2018 Jan 11;2018:8694579. doi: 10.1155/2018/8694579

Table 2.

Elution order of the trans- and cis-isomers of diCQAs on different column matrices (phenyl versus alkyl) using aqueous methanol as the eluent post optimization.

Column chemistry
Pinnacle bi-phenyl Raptor bi-phenyl Viva bi-phenyl Phenomenex bi-phenyl Phenomenex phenyl-hexyl Pinnacle C18 Raptor C18 Viva C18 Ultra C18
Elution order of isomers 1,3-diCQA TM M # C TM M # C TM M # C TM M # C TM M # C TM M # C TM M # C TM M # C TM M # C
1,5-diCQA TM CM # TM CM # TM CM # TM M # TM CM T M CTM # M TM # CM TM #
3,4-diCQA M TM # C M TM # C M TM # C TM C M TM # M TCM # M TM # C M TM # C M TM #
3,5-diCQA TCM M # TCM M # TCM M # TM M # T CM M # T CM T CTM # CM M # T
4,5-diCQA M TCM # M TCM # M TCM # TM CM # M TM # M CTM # M TCM # M TCM # M CTM #

T represents the di-trans-isomer; M represents the first eluting mono-cis-isomer; M # represents the second eluting mono-cis-isomer; C represents the di-cis-isomer.