bmrCD is related to translation stress but is not required for sliding. (A) Quantitative RT-PCR of bmrCD transcript of the wild-type (WT) strain in the absence (-) and presence (+) of chloramphenicol (Cm) at the indicated time points, 4 h, 6 h, 12 h, and 24 h. Quantification cycle (Cq) values were normalized to Cq values for gyrB. Fold expression values are reported relative to the value for the 4-h sample in the absence of Cm. (B) The WT NCIB 3610 strain and a bmrCD deletion strain were spotted on the agar plate in the absence or presence of Cm (0.3 µg/ml). Pictures were taken at h 24. Bar, 1 cm.