. 2017 Nov 6;74(12):1492–1501. doi: 10.1001/jamaneurol.2017.2814

Table 2. ROC Analysis of CSF Aβs for Distinguishing Abnormal From Normal Visual Reading Assessments of Flutemetamol F 18–Labeled PET.

Immunoassay^a	AUC (95% CI)	Cutoff (Cutoff for Mixture Modeling Analysis)^b	Youden J Index	Sensitivity	Specificity
Classic Aβ42-INNOTEST	0.92 (0.89-0.95)	≤548.00 (524.00)	0.78	0.96	0.82
Classic INNOTEST Aβ42:Aβ40 ratio	0.92 (0.88-0.95)	≤0.06 (0.05)	0.73	0.91	0.82
Classic INNOTEST Aβ42:T-tau ratio	0.94 (0.91-0.97)^c	≤1.79 (1.23)	0.84	0.96	0.87
Classic INNOTEST Aβ42:P-tau ratio	0.95 (0.92-0.98)^c^,^d	≤10.30 (8.11)	0.83	0.95	0.89
Modified Aβ42-INNOTEST	0.87 (0.83-0.91)^e	≤1091.00 (1129.00)	0.67	0.92	0.74
Modified INNOTEST Aβ42:Aβ40 ratio	0.93 (0.90-0.96)^f	≤0.12 (0.10)	0.79	0.92	0.87
Modified INNOTEST Aβ42:T-tau ratio	0.94 (0.91-0.97)^g	≤3.30 (2.38)	0.83	0.96	0.88
Modified INNOTEST Aβ42:P-tau ratio	0.95 (0.92-0.97)^g	≤19.60 (16.50)	0.82	0.95	0.87
Aβ42-EI	0.88 (0.84-0.92)^e	≤449.00 (479.00)	0.63	0.82	0.80
Aβ42-EI:Aβ40 ratio	0.93 (0.90-0.96)^h	≤0.10 (0.10)	0.81	0.93	0.88
Aβ42-EI:T-tau ratio	0.94 (0.90-0.97)^h	≤1.44 (1.11)	0.81	0.96	0.86
Aβ42-EI:P-tau ratio	0.94 (0.91-0.96)ⁱ	≤9.59 (7.26)	0.81	0.95	0.87
Aβ42-MSD	0.89 (0.85-0.93)^j	≤506.00 (500.00)	0.70	0.94	0.76
Aβ42-MSD:Aβ40 ratio	0.95 (0.93-0.98)^k	≤0.08 (0.09)	0.86	0.96	0.89
Aβ42-MSD:T-tau ratio	0.94 (0.91-0.97)^l	≤1.39 (1.12)	0.85	0.96	0.89
Aβ42-MSD:P-tau ratio	0.95 (0.92-0.98)^k	≤9.89 (7.84)	0.83	0.96	0.87

Abbreviations: Aβ, amyloid β; AUC, area under the curve; CSF, cerebrospinal fluid; EI, EUROIMMUN; MSD, Meso Scale Discovery; PET, positron emission tomography; P-tau, phosphorylated tau; ROC, receiver operating characteristic; T-tau, total tau.

^{^a}

Cerebrospinal fluid levels of T-tau and P-tau were quantified with EUROIMMUN and INNOTEST enzyme-linked immunosorbent assay kits, respectively. These tau values and Aβ42 values from the 5 different assays (classic and modified INNOTESTs, fully automated Lumipulse, EI, and MSD) were used for the calculations of the CSF Aβ42:tau ratios.

^{^b}

Cutoffs for Aβ42 are given in pg/mL.

^{^c}

P ≤ .01 compared with classic INNOTEST Aβ42:Aβ40 ratio.

^{^d}

P ≤ .05 compared with classic Aβ42-INNOTEST level.

^{^e}

P ≤ .001 compared with classic Aβ42-INNOTEST level.

^{^f}

P ≤ .01 compared with modified Aβ42-INNOTEST level.

^{^g}

P ≤ .001 compared with modified Aβ42-INNOTEST level.

^{^h}

P ≤ .01 compared with Aβ42-EI level.

^ⁱ

P ≤ .001 compared with Aβ42-EI level.

^{^j}

P ≤ .01 compared with classic Aβ42-INNOTEST level.

^{^k}

P ≤ .001 compared with Aβ42-MSD level.

^{^l}

P ≤ .05 compared with Aβ42-MSD level.