Figure 4.

Inactivation of mxiC restores effectors secretion in a mxi I^{L 26A} mutant. Proteins of (a) Congo red supernatants (CR Sup.) or of (b) whole‐cell extracts (WCE) of strains: wild‐type (WT), mxiI mutant (mxiI), mxiI mutant‐expressing MxiI (mxiI/MxiI), mxiI mutant expressing the variant MxiI^{L 26A} (mxiI/MxiI^{L 26A}), and the mxiI mxiC double mutant‐expressing MxiI^{L 26A} variant (mxiI mxiC/MxiI^{L 26A}) were analyzed by Western blot using polyclonal antibodies against IpaA, IpaB, MxiC, and IcsB. All experiments were performed at least three times