Table 1.

General characterization of MxiI mutants

Strains	Colony color	Noninductible secretion	CR induction		% Hemolysis	% Invasion
			Translocators	Effectors
M90T	Red	+	+	+	100 ± 0.97	100 ± 3.2
mxiI	White	−	−	−	0.73 ± 0.56	0 ± 1.2
mxiI +	Red	+	+	+	99.25 ± 1.72	104 ± 7.3
mxiC	Hyper‐red	+++	Delayed	+	1.17 ± 0.84	9 ± 1.7
mxiID17A	Red	+	+	+	98.5 ± 2.87	95 ± 4.6
mxiIL26A	White	−	Reduced	Reduced	7.67 ± 1.84	2 ± 1.9
mxiIP55A	Red	+	+	+	135.77 ± 1.22	97 ± 4.6
mxiIP60A	Red	+	+	+	103.86 ± 5.08	92 ± 6.4
mxiIL63A	White	−	−	−	0.79 ± 0.64	2 ± 2.6
mxiIQ67A a	Pink	+a	+a	−a	55.41 ± 8.3	76 ± 5.2
mxiIQ67E	Pink	+	+	−	65.43 ± 8.9	39 ± 2.9
mxiIQ67K	Red	+	+	+	67.16 ± 0.65	41 ± 3.7
mxiIL70A	Red	+	+	+	105.24 ± 7.19	96 ± 1.8
mxiIY73A	Red	+	+	+	101.42 ± 2.34	65 ± 7.2
mxiIS71A	Red	+	+	+	90.34 ± 10.58	101 ± 2.1
mxiIT82R	Hyper‐red	+++	Delayed	+	1.85 ± 1.46	35 ± 3.2
mxiIT82K	Hyper‐red	+++	Delayed	+	3.40 ± 1.69	52 ± 8.4
mxiIT82A	Red	+	+	+	87.32 ± 8.99	92 ± 6.2
mxiIT82E	Red	+	+	+	71.98 ± 9.95	95 ± 5.3

In blank: residues mutated by site‐directed mutagenesis based on the homology between MxiI and MxiH. In grey: residues mutated by random mutagenesis on mxiI and harboring a mxiC‐like phenotype.

CR, Congo red.

^aCherradi et al. (2013).