| Antibodies |
| TLR4 |
Santa Cruz Biotechnology |
Cat# sc-293072; RRID: AB_10611320 |
| Actin |
Santa Cruz Biotechnology |
Cat# sc-7210; RRID: AB_2223518 |
| SOX2 |
R&D Systems |
Cat# mAb2018; RRID: AB_358009 |
| RBBP5 |
Cell Signaling |
Cat# 13171 |
| GFAP |
Invitrogen |
Cat# NB300-141 |
| TBK1 |
Cell Signaling |
Cat# 3504; RRID: AB_2255663 |
| phospho-TBK1 |
Cell Signaling |
Cat# 5483 |
| IRAK |
Abcam |
Cat# ab238; RRID: AB_303024 |
| phospho-IRAK |
Abcam |
Cat# ab139739 |
| MLL |
Bethyl Laboratories |
Cat# A300-374A; RRID: AB_345243 |
| OCT4 |
Cell Signaling |
Cat# 2750; RRID: AB_823583 |
| NANOG |
Cell Signaling |
Cat# 4903 |
| TLR4-PE |
Santa Cruz Biotechnology |
Cat# sc-13593 PE; RRID: AB_628366 |
| Chemicals, Peptides, and Recombinant Proteins |
| CpG |
InvivoGen |
Cat# tlrl-2216 |
| Pam3C |
InvivoGen |
Cat# tlrl-pms |
| PolyI:C |
InvivoGen |
Cat# tlrl-pic |
| CLI-095 |
InvivoGen |
Cat# tlrl-cli95 |
| BX795 |
InvivoGen |
Cat# tlrl-bx7 |
| LPS |
Sigma |
Cat# L2880 |
| HMGB1 |
GenScript |
Cat# Z02803 |
| Geltrex |
Life Technologies |
Cat# A1413201 |
| Lipofectamine 2000 |
Life Technologies |
Cat# 11668019 |
| Critical Commercial Assays |
| Cell Titer Glo |
Promega |
Cat# G7572 |
| RIPA lysis buffer |
Santa Cruz Biotechnology |
Cat# sc-24948 |
| BCA Protein Assay |
Pierce Biotechnology |
Cat# 23225 |
| ECL Plus Western Blotting Substrate |
Pierce Biotechnology |
Cat # 32132 |
| Papain Dissociation Kit |
Worthington Biochemical |
Cat# LK003150
|
| Secrete-Pair Gaussia Luciferase |
Genecopoeia |
Cat# SPGA-G100 |
| Mouse Neural Stem Cell Nucleofector Kit |
Lonza |
Cat# VPG-1004 |
| qSCRIPT cDNA Supermix |
Quanta Biosciences |
Cat# 95048-100 |
| SYBR-Green Mastermix |
SA Biosciences |
Cat# 330523 |
| Experimental Models: Organisms/Strains |
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ |
The Jackson Laboratory |
JAX: 005557 |
| Recombinant DNA |
| psPAX2 |
Addgene |
Plasmid #12260 |
| pMD2.G |
Addgene |
Plasmid #12259 |
| RBBP5 shRNA vector1 |
Sigma-Aldrich Mission |
TRCN0000353567 |
| RBPP5 shRNA vector2 |
Sigma-Aldrich Mission |
TRCN0000369176 |
| pLKO.1 non-targeting vector |
Sigma-Aldrich Mission |
SHC002 |
| RBBP5 vector |
Applied Biological Materials |
LV283511 |
| TLR4 vector |
Applied Biological Materials |
LV335816 |
| Control vector |
Applied Biological Materials |
LV590 |
| REAGENT or RESOURCE |
SOURCE |
IDENTIFIER |
| pcDNA3-YFP |
Addgene |
Plasmid #13033 |
| pcDNA3-TLR4-YFP |
Addgene |
Plasmid #13018 |
| Sequence-Based Reagents |
| qRT-PCR primers (Table S1) |
This paper |
N/A |
| ChIP primer, NANOG Reverse (−220 to −242): GGCT CTATCACCTTAGACCCACC |
This paper |
N/A |
| ChIP primer, NANOG Forward (−288 to −311): GAGAC TGGTAGACGGGATTAACTG |
This paper |
N/A |
| ChIP primer, OCT4 Reverse (−162 to −183): TCCAG GACCTCAGTGCAGGTC |
This paper |
N/A |
| ChIP primer, OCT4 Forward (−234 to −254): CTGCAC TCCAGTCTGGGCAAC |
This paper |
N/A |
| Software and Algorithms |
| Extreme limiting-dilution analysis |
Hu and Smyth, 2009 |
http://bioinf.wehi.edu.au/software/elda/ |
| GlioVis |
Bowman et al., 2016 |
http://gliovis.bioinfo.cnio.es |
