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. 2017 Aug 15;23(3):521–532. doi: 10.1038/mp.2017.134

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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BLA-derived neurospheres contain functional neurons. (a) Several Thy1- YFP+ cells are present in a basolateral amygdala (BLA)-derived neurosphere. Nuclei are stained with DAPI (blue) Scale bar, 50 μm. (b) Higher magnification of a fixed Thy1-YFP+ neuron (green) co-labeled with βIII-tubulin (red). Scale bar, 10 μm (c) An example of a GAD67+ neuron in a BLA-derived neurosphere. (d, e) Whole-cell recordings from YFP+ cells (scale bar, 10 μm), showing that depolarizing current injections evoke small amplitude action potentials (e).

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