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. 2017 Aug 15;23(3):521–532. doi: 10.1038/mp.2017.134

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Copyright © 2018 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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New neurons are generated in the amygdala nuclei in vivo. (a) BrdU-labeled cells (red, arrows) are present in the basolateral amygdala (BLA) in adult mice. CeA: central amygdala (b) An example of BrdU-labeled cells (red) co-expressing the immature neuronal marker DCX (green) in the BLA. All nuclei are labeled with DAPI (blue). (c) An example of DCX-GFP+ cells (green) in the BLA that are co-labeled with BrdU (red). The merged image and orthogonal views are shown on the far right. (d) DCX-GFP−, BrdU+ cells co-labeled with the mature neuronal marker NeuN in the BLA 4 weeks after the last BrdU injection. (e) A BrdU-labeled cell co-expressing DCX-GFP and NeuN. Scale bars, 50 μm in (a) and 10 μm in (b–e).

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