Figure 1.

Activation of mGlu1 triggers the opening of GluD1 channels in HEK cells. (a) Bath application of DHPG (100 μM) induced a slow inward current in cells co-expressing mGlu1 and GluD1, but not in cells expressing either mGlu1 or GluD1 alone. (b) Example of I/V curve of the DHPG-induced current exhibiting a reversal potential around 0 mV and inward rectification at positive potentials. (c,d) The DHPG-induced current was reduced by D-serine (10 mM) and almost abolished by NASPM (100 μM). (e) Co-expression of the dominant-negative GluD1^VR dead pore mutant with mGlu1 and WT GluD1 dose-dependently reduced the DHPG-induced current. (f) Correct targeting of recombinant proteins at the plasma membrane. DHPG, 3,5-dihydroxyphenylglycine; NASPM, naphtyl-acetyl spermine; WT, wild type.