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. 2018 Feb 22;17:30. doi: 10.1186/s12934-018-0878-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Hydrolysis of increasing amounts of starch substrates by NFAmy13A. The products were analyzed by both reducing sugar assay (a) and TLC analysis (b). Different concentrations (1–18 mg/ml) of potato starch, corn starch or wheat starch were incubated with 1 μM enzymes in the presence of 10 mM CaCl₂ at pH 5.5 and 60 °C for 20 h. M1, glucose; M2, maltose; M3, maltotriose; M4, maltotetraose