. 2018 Feb 22;17:30. doi: 10.1186/s12934-018-0878-y

Table 3.

Comparison of enzymatic characteristics among NFAmy13A and previously reported fungal α-amylases

Source fungi	Specificity activity (U/mg)	Optimal pH	Optimal temperature (°C)	Thermostability	Inhibitor	Activator	Expression host	References
Liquor starter (Fungus)	200.4	5–5.5	60	t_1/2: 1.5 h at 60 °C, 0.4 h at 70 °C; in the presence of 0.01 M CaCl₂, t_1/2: 16 h at 60 °C, 0.7 h at 70 °C	Co²⁺, Hg²⁺, SDS	Ca²⁺, Mg²⁺	Pichia pastoris	This study
Aspergillus awamori KT-11	78.9	4	50	ND	Zn²⁺, Cu²⁺, Hg²⁺	Fe²⁺	Native	[38]
Aspergillus flavus var. columnaris	ND	5	50	96% loss of activity in absence of Ca²⁺, 10% loss of activity in presence of 0.01 M CaCl₂ after incubation at 60 °C for 10 min	None	Ca²⁺	Native	[43]
Aspergillus fischerianus	2314.1	4.5	50	t_1/2: 17 min at 45 °C	Cu²⁺	Co²⁺, Mn²⁺, Fe³⁺	Pichia pastoris	[23]
Aspergillus niger CBS 513.88	2.2	5.5–6.5	NA	Lost rapidly at above 35 °C for 10 min	Na⁺	None	Escherichia coli BL21	[21]
Aspergillus niger JGI 24	75	9	30	Around 20% loss of activity after incubation at 30 °C for 50 min	Na⁺, Mg²⁺	Ca²⁺,Co²⁺	Native	[32]
Aspergillus niveus	ND	6.0	65	t_1/2: 20 min at 70 °C	Hg²⁺, Ag⁺, Fe³⁺	Ca²⁺, NH₄⁺, Na⁺, K⁺, Mn²⁺, Mg²⁺	Native	[33]
Aspergillus oryzae ATCC 76080	410	4–5	50	Lost 80% activity after incubation at 50 °C for 30 min	Hg²⁺, DNFB, NBSI	None	Native	[39]
Aspergillus oryzae EMS-18	1987.7	5.0	40	ND	Mg²⁺, Mn²⁺, Na⁺, Zn²⁺, Ni²⁺, Fe²⁺, Cu²⁺, Co²⁺ and Ba²⁺	Ca²⁺	Native	[44]
Aspergillus oryzae S2	5089.3, 6686.3	5.6	50	t_1/2: 60 min at 50 °C	Mg²⁺, Fe²⁺, Ba²⁺, Cu²⁺, Co²⁺, Mo²⁺, and Mn²⁺	Ca²⁺	Native	[48]
Aspergillus tamarii	778.3	4.5–6.5	50–55	t_1/2: 30 min at 70 °C	Pb²⁺, Cu²⁺, Ag⁺, Fe³⁺	None	Native	[53]
Geomyces pannorum	12,800	5.0	40	40% loss of activity after incubation at 50 °C for 10 min	Ni²⁺, EDTA, Methanol, Teween-20/80	Ca²⁺, Mg²⁺	Aspergillus oryzae	[22]
Malbranchea cinnamomea S168 amylose	2240.7	6.5	65	Around 50% loss of activity after incubation at 60 °C for 30 min	Hg²⁺, Cr³⁺	Mn²⁺, Co²⁺	Native	[40]
Paecilomyces variotii	612.5	4	60	t_1/2: 53 min at 60 °C	Cu²⁺, Al³⁺Fe³⁺, EDTA, K⁺, Pb²⁺, Zn²⁺	Ca²⁺, Co²⁺, Mn²⁺	Native	[34]
Pichia burtonii	ND	4	40	20% loss of activity after incubation at 50 °C for 30 min	Cd²⁺, Cu²⁺, Hg²⁺, Al³⁺, and Zn²⁺	None	Native	[41]
Preussia minima EL-14	138	9	25	ND	Na⁺, Mg²⁺	Mn²⁺, Ca²⁺	Native	[31]
Rhizopus oryzae	1123	4–6	60	50% loss of activity after incubation at 60 °C for 5 min	Cu²⁺, Fe²⁺,	Independence of Ca²⁺	Pichia pastoris	[24]
Rhizomucor pusillus GX-3	20,732	5	70	t_1/2: 75 min at 60 °C	ND	ND	Pichia pastoris	[25]
Saccharomycopsis fibuligera KZ, amylose	120–125	5–6	40–50	ND	ND	ND	Saccharomyces cerevisiae	[27]
Sclerotinia sclerotiorum	ND	4	55	Around 80% loss of activity after incubation at 55 °C for 30 min	Cu²⁺, Mn²⁺, Ba²⁺, Fe²⁺	Weakly stimulated by Ca²⁺	Native	[45]
Scytalidium thermophilum	5.6	6.0	60	t_1/2: 12 min at 60 °C	Cu²⁺, Hg²⁺	Independence of Ca²⁺	Native	[42]
Streptomyces megasporus	847.3	6	60	t_1/2: 2 h at 70 °C	Ag⁺, Ba²⁺, Co²⁺, Cu²⁺, EDTA, Hg²⁺, Mg²⁺, Pb²⁺, Zn²⁺	None	Native	[35]
Thermobifida fusca NTU22	ND	7	60	25% loss of activity after incubation at 60 °C for 3 h	ND	ND	Pichia pastoris	[26]
Thermomyces lanuginosus	349	5	70	32 and 94% loss of activity after incubation at 50 °C for 2 and 18 h	Cu²⁺, FE³⁺	Ba²⁺, Ca²⁺, Cd²⁺, Mg²⁺, Mn²⁺	Native	[46]
Thermomyces lanuginosus	ND	5.6	65	At 65 °C (t_1/2: 0.6 h) the enzyme is nearly 8 times more stable in presence of Ca²⁺	None	Ca²⁺	Native	[47]
Thermomyces lanuginosus	ND	5.2	60	t_1/2: 140 min at 60 °C, 10 min at 70 °C	ND	ND	Native	[36]
Talaromyces pinophilus 1–95	673.1	4.0–5.0	55	60% loss of activity after incubation at 55 °C for 1 h	Ag⁺, Cu²⁺, Mn²⁺	Co²⁺, Fe²⁺, Fe³⁺	Native	[49]

ND, not determined