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. 2018 Feb 21;19:158. doi: 10.1186/s12864-018-4544-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Experimental setup. In the plasmid system 4-to 6-mer homopolymers (HPs) were generated and tested using different primer sets. In the CFTR assay, 17 samples with known genotype were tested. This test system was divided into three parts, exons containing no HPs, HP-containing exons and the 2184 insA mutation