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. 2018 Feb 21;18:74. doi: 10.1186/s12906-018-2134-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Immunofluorescence staining showing activities of microgliacytes and astrocytes in Ipsi and Cont DHs of lumbar spinal cord on day 6 and 18 after CCI in rats. a Representative tissue sections of immunofluorescence staining showing Iba-1-labeled microgliacytes (red) and GFAP-labeled astrocytes (green) in the Ipsi DHs of the lumbar spinal cord. In the superior-right corner of each section, the magnified microgliacyte and astrocyte from each dashed square box are shown. b Bar graphs showing the numbers of microgliacytes and mean fluorescence intensity values of GFAP (for astrocytes) in the Ipsi DHs on day 6 and 18 in the sham and EA groups(mean ± SD, n = 6/group). Results showed that in the early period of CCI-induced neuropathic pain, 2 days’ EA suppressed the activation of microgliacytes (not astrocytes), and in the later period, 2 weeks’ EA suppressed the activation of astrocytes. # P < 0.05, vs the CCI group)