Fig. 3.

Synaptic vesicle exocytosis is associated with α-synuclein release. a Schematic illustration of target molecules for the compounds used in the experiment. b α-Latrotoxin (αLTX, 0.5 nM) increased α-synuclein release, which was blocked by 4 mM EGTA. N = 8. c αLTX significantly increased α-synuclein release in the presence or absence of 1 μM TTX, 50 μM AP5 and 10 μM NBQX. N = 16–32. d LDH activities in the media were not altered by indicated pharmacological treatments. N = 16–24. e Cellular α-synuclein levels were not altered by indicate pharmacological treatments. N = 8. mean ± SEM, ***p < 0.001, ****p < 0.0001 using one-way ANOVA