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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Cell Transplant. 2015 Jul 30;24(11):2237–2250. doi: 10.3727/096368915X688894

Figure 6.

Figure 6

sAPPα and Aβ heterodimerization at monocyte’s cell surface. Immunoprecipitation of sAPPα and Aβs in cell culture. (a) Conditioned media of CHO/APPwt and CHO/APPswe cells expressing APP, Aβs, and sAPPα were immunoprecipitated with anti-Aβ17–27 antibody (4G8) followed by WB detection with anti-Aβ1–16 monoclonal antibody (6E10) (left panel) or an anti-APP N-terminal antibody (22C11) (right panel). WB with 6E10 shows both sAPPα and Aβ bands (left panel), but WB with 22C11 only shows sAPPα band (right panel). (b) Further confirmed by immunoprecipitating with anti-APP N-terminal antibody (22C11) and WB detection with 6E10 reveals both sAPPα and Aβ bands.