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. Author manuscript; available in PMC: 2018 Jul 15.
Published in final edited form as: Nat Chem. 2018 Jan 15;10(3):251–258. doi: 10.1038/nchem.2917

Figure 2. Engineered crosslinkers respond to environmental input combinations on the molecular level.

Figure 2

(a) The chemical structure of the E∧P crosslinker includes an MMP-degradable peptide sequence (green), a photolabile oNB moiety (purple), and two flanking azides (pink) for SPAAC-based hydrogel crosslinking. (b) The MALDI-TOF spectra of the E∧P crosslinker after treatment with all unique combinations of enzyme (E), reductive species (R), and light (P) demonstrates correct molecular response followed each input combination. Expected product masses are highlighted in green. (c) In situ oscillatory rheological analysis of hydrogels crosslinked using treated E∧P demonstrates that AND-gated materials require treatment by both relevant inputs to yield changes in bulk material properties.