Figure 6.

Early cleavage and activation of caspase-9 in HAX-1 knockdown lung epithelial cells infected with the H9N2 virus. Increased caspase cleavage and activation at the early stage of infection in HAX-1 knockdown cells. HAX-1 knockdown (HAX-1_kd) and control (scramble) (HAX-1_wt) cells were infected with the H9N2 virus, and the total cell lysates were analyzed by western blot analyses with anti-cleaved caspase-9 (a) and caspase-3 (b) antibodies. The cytosolic fraction of the lysate was also subjected to western blot analysis with an anti-cytochrome c antibody (c).