Skip to main content
. 2018 Feb 15;24:951–960. doi: 10.12659/MSM.906116

Figure 4.

Figure 4

Upregulation of focal adhesion kinase (FAK) by resveratrol (RSV) enhances migration and tube formation of endothelial progenitor cells (EPCs). (A) The protein level of FAK in EPCs treated by RSV or vehicle. (B–D) RSV-induced EPCs were transfected with FAK siRNA or vehicle. (B) Decreased migrated cells in EPCs transfected with FAK siRNA. (n=3, * p<0.05 versus vehicle group). (C) Decreased migration distance in EPCs transfected with FAK siRNA at 24 hours and 48 hours. (n=3, * p<0.05 versus vehicle group). (D) Decreased tube number in EPCs transfected with FAK siRNA (n=3, * p<0.05 versus vehicle group). (E) A schematic representation showing the putative target site of FAK and mutated target site for miR-138 with the seed region and base substitutions (upper). Luciferase report assays were performed on HEK 293 T cells with co-transfection of FAK-UTR-WT or FAK-UTR-MUT. (F) Luciferase assay of the FAK 3′UTR showed increased activity when EPCs were treated with RSV, as compared to control (vehicle).