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. 2017 May 25;10:42–51. doi: 10.1016/j.jot.2017.05.001

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

RT-qPCR analysis of the mRNA expression of biomarkers related to both (A) osteoclast including TRAP, Cathepsin K, NFATc1; and (B) osteoblast including CoL1A2, ALP, Osterix, and OCN. Statistical significance was assessed using two-tailed Student t test; **p < 0.01 versus WT, ***p < 0.001 versus WT, N_WT = 15, N_GPR1^−/− = 6. CoL1A2 = collagen type I alpha 2; OCN = osteocalcin; RT-qPCR = real-time quantitative polymerase chain reaction; TRAP = tartrate-resistant acid phosphatase; WT = wild type.