Figure 7.

Analyses of the mRNA and protein levels of IL-1β, IL-6, and TNF-α in bone tissues and serum from male GPR1^−/− and WT mice (12 months old), respectively. (A) RT-qPCR analysis and (B) enzyme-linked immunosorbent assay (ELISA) analysis. Statistical significance was assessed using two-tailed Student t test; *p < 0.05 versus WT, N_WT = 15, N_GPR1^−/− = 6. GPR1 = G protein-coupled receptor 1; IL = interleukin; RT-qPCR = real-time quantitative polymerase chain reaction; TNF-α = tumour necrosis factor alpha; WT = wild type.