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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Nature. 2017 May 10;545(7654):311–316. doi: 10.1038/nature22077

Figure 5. The CPK-NLP signalling connection.

Figure 5

a, b, Nitrate triggers phosphorylation-dependent mobility shift of NLP7 in transgenic seedlings. 10 mM KCl (K) or KNO3 (N). 10 mM. CIP, calf-intestinal alkaline phosphatase. c, Ca2+ channel blockers diminish nitrate-triggered NLP7 phosphorylation. d, CPK10/30/32 phosphorylate NLP7 in vitro. e, Phosphorylation of NLP7 by CPK10/30/32 is Ca2+-dependent. f, Mass spectrometric analysis of NLP7 phosphorylation at Serine 205. g, Nitrate-induced phosphorylation of NLP7 at Serine 205 is abolished in icpk. 10 mM KCl (K) or KNO3 (N) for 15 min.