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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Nature. 2017 May 10;545(7654):311–316. doi: 10.1038/nature22077

Figure 6. Nitrate-CPK-NLP signalling is crucial in nutrient-growth networks.

Figure 6

a, CPK10/30/32 translocate to the nucleus in response to nitrate. KNO3, 10 mM. Scale bar, 10 μm. NLS-mC, nuclear HY5-mCherry. BF, bright field. The images are representative of 8 protoplasts. b, Phosphorylation of S205 is required for nitrate-triggered nuclear retention of NLP7-GFP. KNO3, 10 mM. Scale bar, 10 μm. The images are representative of 10 protoplasts. c, CPK10KM interacts with NLP7 in the nucleus in the BiFC assay. KNO3, 10 mM. The images are representative of 8 protoplasts. d, Nitrate-triggered nuclear retention of NLP7-GFP is diminished in icpk. KNO3, 10 mM. NLS-Td-T, nuclear Td-Tomato. The images are representative of 8 protoplasts. e, f, NLP7-GFP but not NLP7(S205A)-GFP complements the nlp7 mutant. Scale bar, 1 cm. The images are representative of 20 seedlings. g, Nitrate-CPK-NLP signalling model.