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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Nature. 2017 May 10;545(7654):311–316. doi: 10.1038/nature22077

Extended Data Figure 8. The CPK phosphorylation residue S205 is required for NLP7 nuclear retention triggered by nitrate at the plant root tip.

Extended Data Figure 8

a, CPK-GFPs are not processed in response to nitrate. Proteins from CPK-GFP transfected protoplasts were analysed by immuno-blots with an anti-GFP antibody. b, Confocal image of NLP7-GFP or NLP7(S205A)-GFP in transgenic nlp7-1 complementation plants in response to nitrate. GFP images recorded at 0 min or 8 min after 10 mM KNO3 induction were shown. Scale bar, 100 μm. The experiments were repeated three times with 10 seedlings for each line with consistent results.