Extended Data Figure 9. Nitrate enhancement of proliferation in the lateral root primordia.

a, b, S205 is crucial for NLP7-mediated transcriptional activation of target genes with diverse functions. Genome-wide transcriptional profiling by RNA-Seq was performed with mesophyll protoplasts expressing NLP7-HA or NLP7(S205A)-HA or the control plasmid for 4.5 h. Red, NLP7 target genes identified by both ChIP-chip¹⁸ and DAP-seq⁶⁸; Black, ChIP-chip only; Grey, DAP-seq only. c, Normalized HTSeq read counts of NLP7-activated genes (listed in a,b) from RNA-Seq experiments. Normalized read counts of NLP7-activated genes calculated as the original HTseq counts divided by the normalization factors were extracted after DESeq2 analysis.