Figure 3.

Effects of recombinant KL, Rap, and 3-MA on autophagy in mice with CLP-induced AKI.

Notes: (A) Western blot for LC3-II, LC3-I, and P62. *P<0.05 vs CLP+media. ^#P<0.05 vs sham control. (B) Images of electron microscopy showing engulfment of degraded cytoplasmic components by autophagosomes (red arrows). The sections were observed under a Hitachi H7500 electron microscope (Hitachi Ltd., Tokyo, Japan). Mice received recombinant KL (0.01 mg/kg) 1 hour after CLP. Rap (1 mg/kg) or 3-MA (30 mg/kg) was administered by intraperitoneal injection 2 hours before CLP surgery.

Abbreviations: Rap, rapamycin; 3-MA, 3-methyladenine; CLP, cecal ligation and puncture; AKI, acute kidney injury; CMC, carboxymethyl cellulose.