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. 2018 Feb 19;11:867–881. doi: 10.2147/OTT.S156891

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© 2018 Chen et al. This work is published and licensed by Dove Medical Press Limited

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Effects of recombinant KL, Rap, and 3-MA on endogenous renal KL expression in mice with CLP-induced AKI.

Notes: (A) Western blot for endogenous renal KL expression. *P<0.05 vs CLP+media. (B) Images of IHC for KL. (C) Quantification of IHC staining. *P<0.05 vs CLP+media. Mice received recombinant KL (0.01 mg/kg) 1 hour after CLP. Rap (1 mg/kg) or 3-MA (30 mg/kg) was administered by intraperitoneal injection 2 hours before CLP surgery. Mouse renal tissues were collected 24 hours after CLP. Sections were stained with anti-KL (1:100, Abcam, Cambirdge, UK) at 4°C overnight. Images at 200× magnification were acquired using a biological imaging microscope (BX53; Olympus Corporation, Tokyo, Japan).

Abbreviations: Rap, rapamycin; 3-MA, 3-methyladenine; CLP, cecal ligation and puncture; AKI, acute kidney injury; CMC, carboxymethyl cellulose; IHC, immunohistochemistry.