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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Biochim Biophys Acta. 2017 Oct 12;1862(1):61–70. doi: 10.1016/j.bbagen.2017.10.006

Fig. 2. Influence of geraniin and urolithin A on nuclear receptor- and Nrf2-dependent transactivation.

Fig. 2

(a) HEK293 cells were transiently transfected with a PPARγ expression plasmid, a PPAR-dependent luciferase reporter (PPRE-LUC) construct as well as a plasmid coding for EGFP. Cells were seeded into 96–wells and treated with the indicated concentrations of Uro and Ger as well as 5 μM pioglitazone as positive control (PC) for 24 h. Then cells were lysed and luciferase-derived luminescence as well as EGFP fluorescence (control for cell number and transfection efficiency) were assessed. Data are expressed as the ratio between luminescence/fluorescence of each well and expressed as fold induction compared to the solvent (DMSO) control. (n = 3) (b–d) HEK cells were treated basically as in (a), except for the use of LXRα- (b), LXRβ- (c) and RXRα (d) expression plasmids and the respective luciferase reporter constructs. 1 μM GW3965 and 5 μM retinoic acid served as positive controls (PC) for LXR α or β and RXRα, respectively. (e) Stably transfected CHO-ARE LUC/EGFP cells were seeded in 96-well plates and treated with the indicated concentrations of Uro and Ger as well as 100 nM CDDO-IM as positive control. After 16 h luminescence and fluorescence were recorded in the cell lysates. Data are expressed as the ratio between luminescence/fluorescence of each well and expressed as fold induction compared to the solvent (DMSO) control (n = 3) (*, p < 0.05; vs DMSO control).