Fig. 4. Influence of urolithin A on the mTOR signaling pathway in macrophages.
Murine macrophages were treated with 20 and 40 μM urolithin A (Uro) in the presence and absence of LPS for 2 and 6 h. Cell lysates were prepared and subjected to western blot analysis for (a) phospho AKT (Thr308) and total AKT, for (b) phospho-TSC2 (Thr1462) and total TSC2, and (c) phospho-p70S6K (Ser389) and actin. Representative blots from three experiments and compiled densitometric data (target/loading control) are depicted (*, p < 0.05; vs LPS-treated solvent control).