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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Mol Oral Microbiol. 2017 Dec 15;33(1):89–104. doi: 10.1111/omi.12204

FIGURE 3.

FIGURE 3

(A) Electrophoretic mobility shift assay showed that rPG1660 could bind to the promoter of PG1660. 1, reaction without rPG1660 as control; 2, reaction with addition of rPG1660; 3, reaction with addition of non-labeled promoter as competitor. (B) Reverse transcription– polymerase chain reaction (RT-PCR) of PG1660 showed that PG1660 was induced when Porphyromonas gingivalis was exposed to hydrogen peroxide. 1, 16S gene amplified by using cDNA of W83; 2, 16S gene amplified by using cDNA of W83 stress; 3, PG1660 gene amplified by using cDNA of W83; 4, PG1660 gene amplified by using cDNA of W83 stress. (C) RT-PCR showed that PG1660 and PG1659 are in the same operon. 1, PG1659-1660 gene amplified by using W83 RNA; 2, PG1659-1660 gene amplified by using W83 cDNA; 3, PG1659 gene amplified by using FLL354 cDNA; 4: PG1659 gene amplified by using W83 cDNA