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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Mol Oral Microbiol. 2017 Dec 15;33(1):89–104. doi: 10.1111/omi.12204

FIGURE 6.

FIGURE 6

Electrophoretic mobility shift assay showed that rPG1660 could bind to the promoter of PG0844 (A), PG1459 (B), PG0374 (C), PG1511 (D), and PG2111 (E). For A anad B: 1, reaction without rPG1660 as control; 2, reaction with addition of rPG1660; 3, reaction with addition of non-labeled promoter as competitor. For C, D and E: 1, no protein; 2, 0.5 pmol of rPG1660; 3, 1 pmol of rPG1660; 4, 5 pmol of rPG1660; 5, 1 pmol of rPG1660 and non-labeled promoter DNA. (F) The conservative sequences of promoter region of the genes been regulated by PG1660 containing –35 “kTCrsA” and –10 “bdTAAT” (k:G or T; r: A or G; s: C or G; b: not A; d: not C) motifs