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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: Mol Oral Microbiol. 2010 Nov 18;26(1):62–77. doi: 10.1111/j.2041-1014.2010.00596.x

Figure 2.

Figure 2

(A) Reverse transcription–polymerase chain reaction (RT-PCR) analysis of RNA extracted from Porphyromonas gingivalis W83 and FLL302. Total RNA was extracted from FLL302 (lanes 1–3 and 7–9) and W83 (lanes 4–6 and 10–12) grown to mid log-phase (OD600, 0.6) and subjected to RT-PCR. Porphyromonas gingivalis FLL302: lane 1, primers (P1 and P2, Table 1) plus reverse transcriptase; lane 2, primers (P16 and P17, Table 1) plus reverse transcriptase; lane 3, primers (P14 and P15, Table 1) plus reverse transcriptase; P. gingivalis W83: lane 4, primers (P1 and P2, Table 1) plus reverse transcriptase; lane 5, primers (P16 and P17, Table 1) plus reverse transcriptase; lane 6, primers (P14 and P15, Table 1) plus reverse transcriptase; lanes 7–12, corresponding primer sets for the bcp, recA and vimA genes minus reverse transcriptase (negative controls). All lanes contain 5 μl of the amplified mixture. (B) Location of the primers used in RT-PCR analysis.