Table 2. Rates of midgut infection, dissemination, and oral transmission of Cache Valley virus by Aedes japonicus japonicus mosquitoes after a 2-week extrinsic incubation, Appalachian Region, United States, 2015*.

Replicate	Infectious blood meal titer, log10 PFU/mL	No. tested	Nondisseminated infections, %	Disseminated infections, %	Transmission, %
1	1.2 × 106	18	44	39	33
2	1.6 × 105	26	42	42	27
3	4.6 × 106	30	37	33	27
Total	Not applicable	74	41	38	28

*Virus recovered from the midgut only was classified as a nondisseminated infection. Mosquitoes with virus-positive legs and wings were considered to have a disseminated infection. If virus was detected in salivary expectorate, it was classified as transmitting.