Fig 7. Narasin inhibits mucus secretion in Calu-3 cells.

A. Narasin-treated Calu-3 cells were stained with WGA (green) and Muc5ac (Red). Narasin-treated Calu-3 cells accumulated Muc5ac-positive puncta and WGA-stained glycoproteins in the cytosol. B. The number and average size of WGA stained signal was plotted graphically. C. The number of Muc5ac stained puncta was measured and represented graphically. D. Although the PERK level was slightly increased, ER stress was not strongly induced by narasin in Calu-3 cells. Thapsigargin induced strong ER stress response but mucus secretion was not affected. E. Narasin did not severely induce ER stress responses in Xenopus embryos. P-values were calculated from student’s t-test using the triplicate Q-PCR data set. A.U.; Arbitrary unit.