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. 2018 Feb 22;13(2):e0193210. doi: 10.1371/journal.pone.0193210

Fig 6. Immunomodulatory effects of α7nAChR agonist nicotine on LPS activated mouse splenocytes is reversed by mPGES-1 gene deletion.

Fig 6

Primary splenocyte cultures of mPGES-1(+/+) and (-/-) mice were pretreated with nicotine (100 μM) for 30 mins and later activated with the endotoxin, LPS (10ng/ml). Cell supernatants were analyzed for LPS induced cytokine production following 3 hour incubation. (a) TNFα as measured in cell culture supernatants by ELISA. (*p<0.05; LPS versus LPS+Nicotine within WT; One-way ANOVA, n.s. p>0.05; WT versus KO within LPS+Nicotine treatment group; One-way ANOVA). (b) Fold change of TNFα, KC Gro and IL-1β as measured in cell culture supernatants by Multiplex assay (*p<0.05; LPS versus LPS+Nicotine within WT; One-way ANOVA). Each sample was run as duplicates during ELISA and values are represented as mean ±SEM from 3 independent experiments. Due to high variations between individual experiments, cytokine production in each group was normalized to TNF α level induced by LPS and represented as % fold change.