Fig 1. Tb927.6.3050 serves as a specific potency determinant for aminomethylphenoxy benzoxaboroles.

(A) Tb927.6.3050 identification in RIT-seq from a genome-scale screening for potency determinants of benzoxaboroles. The gene locus is indicated in red, with flanking genes in black, in the relevant chromosomal context. Each peak represents an identification by sequencing and the relative height corresponds to the number of reads. A pair of short sequences were introduced in the original library [26], flanking the individual RNAi targeting fragment in both directions, as a unique bar code to ensure sequencing specificity. The corresponding identifications are indicated in color, red for the forward sequence and blue for the reverse, distinguished from all other identification indicated in grey. The aminomethylphenoxy moieties in the respective compounds are indicated with the dotted rectangles. (B) The changes in potency (EC₅₀) of chemically diverse benzoxaboroles upon Tb927.6.3050 RNAi.