Fig 3. Hic1 expression in T cells and dendritic cells is not required for immunity to Citrobacter rodentium infection.
(A–C) Hic1CD4 and Hic1fl/fl mice were orally inoculated with C. rodentium. (A) Weight loss (percentage of initial weight) was calculated for each mouse over course of infection. (B) Bacterial loads (CFU/g) from fecal pellets were measured over course of infection. (C) Quantitative RT-PCR was performed to determine expression of Il17a, Il22 and Reg3g from distal colon tissue 14 days post inoculation. (D–F) Hic1CD11c and Hic1fl/fl mice were orally inoculated with C. rodentium. (D) Weight loss (percentage of initial weight) was calculated for each mouse over course of infection. (E) Bacterial loads (CFU/g) from fecal pellets were measured over course of infection. (F) Quantitative RT-PCR was performed to determine expression of Il17a, Il22 and Reg3g from distal colon tissue 11 days post inoculation. (A-C) Data are pooled from 3 independent experiments (n = 7–11 per group). (D-F) Data are pooled from 2 independent experiments (n = 4–5 per group) *, P < 0.05; Mann-Whitney test. Error bars indicate SEM. ns, not significant.