Fig 8. Recombinant mouse IL-22 is sufficient to promote resistance to Citrobacter rodentium in Hic1Rorc mice.
(A-D) Intestinal ILC3s from Hic1Rorc and Hic1fl/fl mice were analysed for intracellular IL-22 by flow cytometry at steady state (A, B) or at day 4 post infection with C. rodentium (C, D). (E–G) Hic1Rorc mice were infected with C. rodentium and treated with or without rmIL-22. (E) Weight loss (percentage of initial weight) was calculated for each mouse over course of infection. H&E stained histological sections of colon (F) and liver (G) from 11 days post infection. Scale bar represents 100μm. Black arrows indicate inflammatory infiltrate. (A–D) Data are from 2 independent experiments (n = 4 per group) (E) Data are from 2 independent experiments (n = 5–6 mice per group). *, P < 0.05; Mann-Whitney test. Error bars indicate SEM.